Autolysosomal b-catenin degradation regulates Wnt-autophagy-p62 crosstalk
نویسندگان
چکیده
Katy J Petherick, Ann C Williams, Jon D Lane, Paloma Ordóñez-Morán, Joerg Huelsken, Tracey J Collard, Helena JM Smartt, Jennifer Batson, Karim Malik, Chris Paraskeva* and Alexander Greenhough* Cancer Research UK Colorectal Tumour Biology Group, School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK, Cell Biology Laboratories, School of Biochemistry, University of Bristol, Bristol, UK, Cancer Stem Cell Laboratory, Ecole Polytechnique Fédérale de Lausanne, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland, School of Physiology and Pharmacology, University of Bristol, Bristol, UK and Cancer Epigenetics Laboratory, School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK
منابع مشابه
Autolysosomal β-catenin degradation regulates Wnt-autophagy-p62 crosstalk.
The Wnt/β-catenin signalling and autophagy pathways each play important roles during development, adult tissue homeostasis and tumorigenesis. Here we identify the Wnt/β-catenin signalling pathway as a negative regulator of both basal and stress-induced autophagy. Manipulation of β-catenin expression levels in vitro and in vivo revealed that β-catenin suppresses autophagosome formation and direc...
متن کاملMalin regulates Wnt signaling pathway through degradation of dishevelled2.
Using yeast-two hybrid screening followed by co-immunoprecipitation assay, we have found that the Lafora disease ubiquitin ligase malin interacts with dishevelled2, a key mediator of Wnt signaling pathway. Overexpression of malin enhances the degradation of dishevelled2 and inhibits Wnt signaling, which is evident from the down-regulation of β-catenin target genes and the decrease in β-catenin-...
متن کاملBAMBI Is Expressed in Endothelial Cells and Is Regulated by Lysosomal/Autolysosomal Degradation
BACKGROUND BAMBI (BMP and Activin Membrane Bound Inhibitor) is considered to influence TGFβ and Wnt signaling, and thereby fibrosis. Surprisingly data on cell type-specific expression of BAMBI are not available. We therefore examined the localization, gene regulation, and protein turnover of BAMBI in kidneys. METHODOLOGY/PRINCIPAL FINDINGS By immunofluorescence microscopy and by mRNA expressi...
متن کاملInterplay between Leucine-Rich Repeat Kinase 2 (LRRK2) and p62/SQSTM-1 in Selective Autophagy
The deposit of polyubiquitinated aggregates has been implicated in the pathophysiology of Parkinson's disease (PD), and growing evidence indicates that selective autophagy plays a critical role in the clearance of ubiquitin-positive protein aggregates by autophagosomes. The selective autophagic receptor p62/SQSTM-1, which associates directly with both ubiquitin and LC3, transports ubiquitin con...
متن کاملActivation of Wnt signaling reduces high-glucose mediated damages on skin fibroblast cells
Objective(s): High-glucose (HG) stress, a mimic of diabetes mellitus (DM) in culture cells, alters expression of a large number of genes including Wnt and NF-κB signaling-related genes; however, the role of Wnt signaling during HG-mediated fibroblast damage and the relationship between Wnt and NF-κB signaling have not been understood. In this study, we aimed to investigate the ffects of Wnt sig...
متن کامل